• P-ISSN2233-4203
  • E-ISSN2093-8950

Article Detail

Home > Article Detail
  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Analysis of Glycerol with Isolation of Endogenous Interferences using “Dilute and Shoot” Strategy and High-Resolution Mass Spectrometry in Human Urine for Antidoping Testing

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2016, v.7 no.4, pp.111-115
https://doi.org/10.5478/MSL.2016.7.4.000
Kim Yongseok (Korea Institute of Science and Technology)
Min Hophil (Doping Control Center, Korea Institute of Science and Technology)
Sung Changmin (Korea Institute of Science and Technology)
Park Ju-Hyung (Korea Institute of Science and Technology)
Son Junghyun (Korea Institute of Science and Technology)
Lee Kang Mi (Korea Institute of Science and Technology)
Kim Ho Jun (Korea Institute of Science and Technology)
Lee Jaeick (Korea Institute of Science and Technolog)
Kwon Oh Seung (Korea Institute of Science and Technolog)
Kim Ki Hun (Korea Institute of Science and Technolog)
  • Downloaded
  • Viewed

Abstract

Glycerol was identified and isolated from endogenous interferences during analysis of human urine using high-resolution mass spectrometry (HRMS) for doping control. Urinary sample preparation was simple; the samples were diluted with an organic solvent and then analyzed using a liquid chromatography-mass spectrometry (“dilute and shoot” method). Although the interfering ion peaks were observed at the similar retention time of glycerol, the inference could be identified by isolation with HRMS and further investigation. Thus, creatinine was identified as the endogenous interference for glycerol analysis and it also caused ion suppression resulting in the decrease of glycerol signal. This study reports the first identification and efficient isolation of endogenous interferences in human urine for “dilute and shoot” method. The information about ion suppression could be novel to prevent overestimation or a false result for antidoping analysis.

keywords
Mass spectrometry Doping control Dilute and shoot Orbitrap Glycerol analysis


Reference

1

WADA. (2016). The 2016 prohibited list international standard. https://www.wada-ama.org/en/international-standards.

2

Polyviou, T. P.. (2012). . Nutr, 4, 1171-.

3

Nash, T.. (1953). . Biochem. J, 55, 416-.

4

Thevis, M.. (2008). . Eur. J. Mass Spectrom, 14, 117-.

5

Dong, Y.. (2016). . J. Chromatogr. B, 1022, 153-.

6

Dong, Y.. (2014). . J. Chromatogr. B, 957, 30-.

7

Jeong, E. S.. (2015). . Rapid Commun. Mass Spectrom, 29, 367-.

8

Görgens, C.. (2015). . Anal. Bioanal. Chem, 407, 5365-.

9

Jiménez Girón, A.. (2012). . Anal. Chim. Acta, 721, 137-.

10

Guddat, S.. (2011). . Drug Test. Anal, 3, 836-.

11

Fitzgerald, R. L.. (2012). . J. Anal. Toxicol, 36, 106-.

12

Beck, O.. (2014). . Bioanalysis, 6, 2229-.

13

Annesley, T. M.. (2003). . Clin. Chem, 49, 1041-.

14

Schlittenbauer, L.. (2016). . Rapid Commun. Mass Spectrom, , 1560-.

Submission Date
2016-11-30
Revised Date
2016-12-14
Accepted Date
2016-12-19
상단으로 이동

Mass Spectrometry Letters