• P-ISSN2233-4203
  • E-ISSN2093-8950

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Optimization of Solid Phase Extraction Method for Quantitative Analysis of Perfluorooctanoic Acid in Serum using Liquid Chromatography-Tandem Mass Spectrometry

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2014, v.5 no.1, pp.30-33
https://doi.org/10.5478/MSL.2014.5.1.30
Lee Hyun-Seok (Korea Research Institute of Standard and Science)
Yim Yong-Hyeon (Korea Research Institute of Standard and Science)
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Abstract

A solid phase extraction (SPE) method was optimized for the quantitative analysis of perfluorooctanoic acid (PFOA)in serum using hydrophilic-lipophilic balance SPE and LC-MS/MS. Fetal bovine serums spiked with 13C8-PFOA before or afterSPE were used as test samples for evaluation of the SPE efficiency. Simultaneous evaluation of matrix effects and absolute SPErecovery for 13C8-PFOA in serum using different sample pre-treatments and SPE conditions allowed optimization of SPE processefficiency with minimal matrix effect and decent SPE recovery. Introduction of protein precipitation as a sample pre-treatmentprocedure for serum samples before SPE generally decreased matrix effect in LC-MS/MS analysis and provided morestable recovery of PFOA.

keywords
perfluooctanoic acid solid phase extraction fatal bovine serum LC-MS/MS


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Submission Date
2014-02-15
Revised Date
2014-03-11
Accepted Date
2014-03-12
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Mass Spectrometry Letters