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- E-ISSN 2093-8950
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- E-ISSN2093-8950
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Enhanced Detection of Glycans by MALDI-TOF Mass Spectrometry Using a Binary Matrix of 2,5-Dihydroxybenzoic Acid and 2,6-Dihydroxybenzoic Acid
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2013, v.4 no.2, pp.38-40
https://doi.org/10.5478/MSL.2013.4.1.38
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
Kim,
J., Kim,
T., Lee,
J., Im,
H., &
Kim,
J.
(2013). Enhanced Detection of Glycans by MALDI-TOF Mass Spectrometry Using a Binary Matrix of 2,5-Dihydroxybenzoic Acid and 2,6-Dihydroxybenzoic Acid. Mass Spectrometry Letters, 4(2), 38-40, https://doi.org/10.5478/MSL.2013.4.1.38
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Abstract
Glycans released from ovalbumin by PNGase F were analyzed by matrix-assisted laser desorption/ionization time-offlight(MALDI-TOF) mass spectrometry using three different dihydroxybenzoic acid (DHB) matrix systems: 2,5-DHB, 2,6-DHB, and a 2,5-DHB/2,6-DHB binary matrix. Relative to the results obtained with the single-component matrices (2,5-DHB or2,6-DHB), the 2,5-DHB/2,6-DHB binary matrix boasted lower background noise and higher sensitivity. A total of 16 glycanpeaks were observed using the 2,5-DHB/2,6-DHB binary matrix, while only 10 and 9 glycan peaks were observed using the 2,5-DHB and 2,6-DHB matrices, respectively.
- keywords
- Glycans, MALDI, PNGase F, ovalbumin, oligosaccharides, dihydroxybenzoic acid
Reference
1
An, H. J. (2003). . Anal. Chem, 75, 5628-.
2
Serenus Hua. (2013). Multi-Level Characterization of Protein Glycosylation. Mass Spectrometry Letters, 4(1), 10-17.
3
Harvey, D. J. (1993). . Rapid Commun. Mass Spectrom, 7, 614-.
4
Aera Lee. (2009). Effect of Dihydroxybenzoic Acid Isomers on the Analysis of Polyethylene Glycols in MALDI-MS. Bulletin of the Korean Chemical Society, 30(5), 1127-1130.
5
Shin, S. (2011). . Anal. Biochem, 414, 125-.
6
Park, E. (2012). . Food Chem, 134, 1658-.
7
Jessome, L. (2008). . Rapid Commun. Mass Spectrom, 22, 130-.
8
Laugesen, S. (2003). . J. Am. Soc. Mass Spectrom, 14, 992-.
9
Lastovickova, M. (2009). . Int. J. Mass Spectrom, 281, 82-.
10
Harvey, D. J. (2000). . J. Am. Soc. Mass Spectrom, 11, 564-.
11
Snovida, S. I. (2008). . J. Am. Soc. Mass Spectrom, 19, 1138-.
12
Kjellstrom, S.. (2004). . Anal. Chem, 76, 5109-.
13
Park, S. (2013). . Rapid Commun. Mass Spectrom, 27, 842-.
- Submission Date
- 2013-06-23
- Revised Date
- 2013-06-26
- Accepted Date
- 2013-06-27
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