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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Examination of Various Metal Ion Sources for Reducing Nonspecific Zinc finger−Zn2+ Complex Formation in ESI Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2012, v.3 no.3, pp.82-85
https://doi.org/10.5478/MSL.2012.3.3.82
Park Soojin (Sogang University)
Park Sunhee (Sogang University)
Oh Joo Yeon (Korea Research Institute of Standards and Science)
Han Sang Yun (Korea Research Institute of Standards and Science)
Jo Kyubong (Sogang University)
Oh Han Bin (Sogang University)
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Abstract

The formation of zinc finger peptide−Zn2+ complexes in electrospray ionization mass spectrometry (ESI-MS) wasexamined using three different metal ion sources: ZnCl2, Zn(CH3COO)2, and Zn(OOC(CHOH)2COO). For the four zinc fingerpeptides (Sp1-1, Sp1-3, CF2II-4, and CF2II-6) that bind only a single Zn2+ in the native condition, electrospray of apo-zinc fingerin solution containing ZnCl2 or Zn(CH3COO)2 resulted in the formation of zinc finger−Zn2+ complexes with multiple zincions. This result suggests the formation of nonspecific zinc finger−Zn2+ complexes. Zn(tartrate), Zn(OOC(CHOH)2COO),mainly produced specific zinc finger−Zn2+ complexes with a single zinc ion. This study clearly indicates that tartrate is an excellentcounter ion in ESI-MS studies of zinc finger−Zn2+ complexes, which prevents the formation of nonspecific zinc finger−Zn2+complexes.

keywords
Zinc finger, Zinc ion, Electrospray ionization, Tartrate, Noncovalent complexes


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Submission Date
2012-08-31
Revised Date
2012-09-17
Accepted Date
2012-09-17
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