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- E-ISSN 2093-8950
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- E-ISSN2093-8950
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Simultaneous Quantification of Oleins (triolein, diolein and monoolein) in Mouse Feces using Liquid Chromatography-Electrospray Ionization/Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2012, v.3 no.3, pp.68-73
https://doi.org/10.5478/MSL.2012.3.3.68
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technolog)
(Korea Institute of Science and Technology)
Lim,
J. H., Lee,
J., Jang,
Y., Chung,
H., Lee,
W., &
Chung,
B.
C.
(2012). Simultaneous Quantification of Oleins (triolein, diolein and monoolein) in Mouse Feces using Liquid Chromatography-Electrospray Ionization/Mass Spectrometry. Mass Spectrometry Letters, 3(3), 68-73, https://doi.org/10.5478/MSL.2012.3.3.68
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Abstract
Fat malabsorption is an important cause of poor growth in infancy and childhood. Steatorrhea tests have been developedusing various methods. Traditional measurements of stool fat, however, require large samples and it often takes as a week tocomplete the analysis. In this paper, a liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) methodwas developed for simultaneous quantitative analysis of triacylglycerols, triolein, diolein and monoolein, in mouse feces. Moreover,the procedure was rapid, simple as well as compatible with LC-ESI/MS. Chloroform-isopropyl alcohol solution was usedfor fat-soluble sample extraction. After centrifugation and filtration, an analytical solution was prepared. Triolein, diolein andmonoolein were separated using non-aqueous reversed-phase column with the mobile phase consisting of A (methanol) and B(acetone-isopropyl alcohol). The precision (% CV) and accuracy (% bias) of the assay were 3.8-14.7% and 85.2-114.9%, respectively. This method has been successfully applied to simultaneous determination of triolein, diolein and monoolein in feces from30 mice. This method can therefore be applied to measure triacylglycerols in mouse feces accurately and precisely by LC-ESI/MS, thereby helping to predictive biomarker in fat malabsorption and diagnostic research.
- keywords
- Triacylglycerols, Steatorrhea, Liquid chromatography-electrospray ionization/mass spectrometry, Mouse feces
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- Submission Date
- 2012-08-27
- Revised Date
- 2012-09-10
- Accepted Date
- 2012-09-10
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