• P-ISSN2233-4203
  • E-ISSN2093-8950

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Microwave-assisted Protein Digestion on Various Locations of a Microplate

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.4, pp.84-87
https://doi.org/10.5478/MSL.2011.2.4.084
Lee Ji Hye (Chungnam National University)
Park Sehwan (Chungnam National University)
Lee Sunyoung (Chungnam National University)
Hong Jangmi (Chungnam National University)
Park Kyu Hwan (Korea Basic Science Institute)
Hyun Sik Kim (Korea Basic Science Institute)
Kim Jeongkwon (Chungnam National University)
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Abstract

The effectiveness of microwave-assisted protein digestion in different well positions of a 96-well microplate wasinvestigated where microwave-assisted protein digestion of bovine serum albumin was performed in 10 different wells of a 96-wellmicroplate in a microwave oven. Similarly increased sequence coverages (~70%) were generally observed for the 10 microwaveassistedprotein digestion samples compared to conventional overnight digestion (63%), which is possibly due to higher miscleavageratios (~53%) of the samples from microwave-assisted protein digestion than conventional overnight digestion (42.1%). The reproducibleresults of microwave-assisted digestions from different well positions demonstrate the potential of high-throughput analysis ofproteins using microwave-assisted protein digestion.

keywords
Microwave Mass spectrometry MALDI Microplate Digestion


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Submission Date
2011-12-09
Revised Date
2011-12-13
Accepted Date
2011-12-14
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