• P-ISSN2233-4203
  • E-ISSN2093-8950

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Development of a Matrix-prespotted Plate for Enhancing the Reproducibility of Serum Glycan Analysis by MALDI-TOF-MS

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.3, pp.61-64
https://doi.org/10.5478/MSL.2011.2.3.061
Ha Miyoung (Applied Surface Technology Inc.)
In Youngha (Applied Surface Technology Inc.)
Maeng Hyesun (Applied Surface Technology Inc.)
Zee Ok Pyo (Sungkyunkwan University)
Lee Jongsik (Applied Surface Technology Inc.)
Kim Yangsun (Applied Surface Technology Inc.)
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Abstract

Matrix Assisted Laser Desorption/Ionization-Time-of-Flight mass spectrometry (MALDI-TOF-MS) is the mostwidely used MS technique for glycan analysis. However, the poor point-to-point and sample-to-sample reproducibility becomes alimit in glycan biomarker research. A prespotted MALDI plate which overcomes the large crystal formation of 2,5-dihydroxybenzoicacid (DHB) has been developed and applied for glycan analysis. A homogeneous matrix coated surface without a crystal structurewas formed on a hydrophilic/ hydrophobic patterned surface using a piezoelectric device. The reproducible MALDI-TOF-MSdata have been presented using MALDI imaging of beer glycan as well as serum glycan eluted from 10% and 20% ACN elutionfractions. The glycan profile from the serum glycan by MALDI-TOF-MS with a DHB prespotted plate was highly conserved for10 different spectra and the coefficient of variations of significant ion peaks of MALDI data varies from 3.59 to 19.95.

keywords
MALDI-TOF-MS Glycan analysis Prespotted MALDI plate


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Submission Date
2011-09-09
Revised Date
2011-09-14
Accepted Date
2011-09-14
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