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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Enrichment of Peptides using Novel C8-functionalized Magnetic Nanoparticles for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometric Analysis

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.2, pp.53-56
https://doi.org/10.5478/MSL.2011.2.2.053
Song Sunmi (Chungnam National University)
Yang Hyo-Jik (Chungnam National University)
Kim Jin Hee (Chungnam National University)
Shin Seongjae (Chungnam National University)
Park Eun Hye (Chungnam National University)
Kim Jeongkwon (Chungnam National University)
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Abstract

C8-functionalized magnetic nanoparticles were synthesized by coating magnetic Fe3O4 nanoparticles with silica–amine groups using 3-aminopropyltriethoxysilane and by subsequently modifying the amine groups with chloro(dimethyl)octylsilaneto produce octyl groups on the surface of the MNPs. The C8-functionalized MNPs were used to enrich peptides from trypticprotein digests of myoglobin and α-casein. The enriched peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI−ΤΟF−MS). MALDI-MS was also used to investigate desalting of the C8-functionalized MNPs. Sample solutions were prepared in 1.0 M NaCl, and the successful removal of salt was observed. Enrichmentwith C8-functionalized MNPs was very effective for separating and concentrating tryptic peptides.

keywords
3-aminopropyltriethoxysilane, chloro(dimethyl)octylsilane, MALDI, magnetic nanoparticles


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Submission Date
2011-05-27
Revised Date
2011-06-07
Accepted Date
2011-06-07
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