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- E-ISSN 2093-8950
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Validation and Applications of Gas Chromatography-Combustion/isotope Ratio Mass Spectrometric Method to Control Misuse of Androgens in Human
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.2, pp.33-36
https://doi.org/10.5478/MSL.2011.2.2.033
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Hanyang University)
(Korea Institute of Science and Technology)
(Korea Institute of Science & Technology)
(Inje University)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Hanyang University)
(Korea Institute of Science and Technology)
(Korea Institute of Science & Technology)
(Inje University)
(Korea Institute of Science and Technology)
Lee,
K. M., Kim,
H. J., Jeong,
E. S., Yoo,
H. H., Kwon,
O., Jin,
C., Kim,
D. H., &
Lee,
J.
(2011). Validation and Applications of Gas Chromatography-Combustion/isotope Ratio Mass Spectrometric Method to Control Misuse of Androgens in Human. Mass Spectrometry Letters, 2(2), 33-36, https://doi.org/10.5478/MSL.2011.2.2.033
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Abstract
The misuse of anabolic androgenic steroids is of particular concern in sports and society. Thus, it is of great importance todiscriminate endogenous steroids such as testosterone or testosterone prohormones from their chemically identical syntheticcopies. In this study, gas chromatography-combustion/isotope ratio mass spectrometric (GC−C/IRMS) method has been developedand validated for discriminating the origin of anabolic androgenic steroids. The method involves the solid-phase extraction,enzymatic hydrolysis with β-glucuronidase, HPLC-fractionation for the cleanup and analysis by GC−C/IRMS. The difference(Δ13C) of urinary δ13C values between synthetic analogues and endogenous reference compounds (ERC) by GC−C/IRMS wasused to elucidate the origin of steroids, and intra- and inter-day precision, specificity and isotope fractionation were evaluated. The present GC−C/IRMS method combined with HPLC cleanup was accurate and reproducible enough to be successfullyapplied to the test of urine sample from suspected anabolic steroid abusers.
- keywords
- Steroids, Endogenous, Validation, GC-C/IRMS
Reference
1
Cawley, A. T. (2009). . V. Steoids, 74, 379-.
2
The World Anti-Doping Agency. Prohibited List.
3
Chung, B. C. (1990). . J. Anal. Toxicol, 14, 91-.
4
Coppen, J. J. W.. (1979). . Trop. Sci, 21, 125-.
5
Ueki, M. (1999). . Rapid Commun. Mass Spectrom, 13, 2237-.
6
Becchi, M. (1994). . Rapid Commun. Mass Spectrom, 8, 304-.
7
Aguilera, R. (1996). . J. Mass Spectrom, 31, 169-.
8
Shackleton, C. H. L.. (1997). . Steoids, 62, 379-.
9
Cawley, A. T. (2008). . J. Mass Spectrom, 43, 854-.
10
Mareck, U. (2008). . J. Mass Spectrom, 43, 877-.
11
Shackleton, C. H. L. (1997). . Steoids, 62, 665-.
12
Aguilera, R. (1999). . J. Chromatogr. B, 727, 95-.
13
Aguilera, R. (2001). . Clin.Chem, 47, 292-.
14
Piper, T. (2008). . Rapid Commun. Mass Spectrom, 22, 2161-.
15
Saudan, C. (2009). . J.Chromatogr. B, 877, 2321-.
- Submission Date
- 2011-03-03
- Revised Date
- 2011-03-24
- Accepted Date
- 2011-06-08
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