• P-ISSN2233-4203
  • E-ISSN2093-8950

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

LC-MS/MS-based Proteomic Analysis of Locally Advanced Rectal Tumors to Identify Biomarkers for Predicting Tumor Response to Neoadjuvant Chemoradiotherapy

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2022, v.13 no.3, pp.84-94
https://doi.org/10.5478/MSL.2022.13.2.84
Kim Kyung-Ok (Gachon University)
Duong Van-An (Gachon University)
Han Na-Young (Gachon University)
Park Jong-Moon (Gachon University)
Kim Jung Ho (Gachon University)
Lee Hookeun (Gachon University)
Baek Jeong-Heum (Gachon University)
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Abstract

Neoadjuvant chemoradiotherapy (nCRT) is a standard therapy used for locally advanced rectal cancer prior to sur- gery, which can more effectively reduce the locoregional recurrence rate and radiation toxicity compared to postoperative chemoradiotherapy. The response of patients to nCRT varies, and thus, robust biomarkers for predicting a pathological complete response are necessary. This study aimed to identify possible biomarkers involved in the complete response/non-response of rec- tal cancer patients to nCRT. Comparative proteomic analysis was performed on rectal tissue samples before and after nCRT. Pro- teins were extracted for label-free proteomic analysis. Western blot and real-time PCR were performed using rectal cancer cell line SNU-503 and radiation-resistant rectal cancer cell line SNU-503R80Gy. A total of 135 up- and 93 down-regulated proteins were identified in the complete response group. Six possible biomarkers were selected to evaluate the expression of proteins and mRNA in SNU-503 and SNU-503R80Gy cell lines. Lyso-phosphatidylcholine acyltransferase 2, annexin A13, aldo-ketose reductase family 1 member B1, and cathelicidin antimicrobial peptide appeared to be potential biomarkers for predicting a pathological complete response to nCRT. This study identified differentially expressed proteins and some potential biomarkers in the complete response group, which would be further validated in future studies.

keywords
LC-MS/MS; proteomics; neoadjuvant chemoradiotherapy; locally advanced rectal cancer; biomarker


Submission Date
2022-07-19
Revised Date
2022-08-12
Accepted Date
2022-08-19
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Mass Spectrometry Letters