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- E-ISSN 2093-8950
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- E-ISSN2093-8950
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Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.4, pp.137-145
https://doi.org/10.5478/MSL.2021.12.4.137
(Korea Food Research Institute)
(Korea University)
(Korea University)
(Korea University)
(Korea University)
(Korea University)
(Korea University)
Lee,
H. H. L., Chae,
S. Y., Son,
M. K., &
Kim,
H.
I.
(2021). Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase. Mass Spectrometry Letters, 12(4), 137-145, https://doi.org/10.5478/MSL.2021.12.4.137
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Abstract
Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS com-bined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-con-taining peptide ions.
- keywords
- ion mobility mass spectrometry, tryptic peptide, ion chemistry, glutamine
- Submission Date
- 2021-10-28
- Revised Date
- 2021-11-30
- Accepted Date
- 2021-11-30
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