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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Analysis of Low Molecular Weight Collagen by Gel Permeation Chromatography

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.3, pp.81-84
https://doi.org/10.5478/MSL.2021.12.3.81
Yoo Hee-Jin (Korea Basic Science Institute (KBSI))
Kim Duck-Hyun (Korea Basic Science Institute (KBSI))
Park Su-Jin (Chungnam National University)
Cho Kun (Korea Basic Science Institute (KBSI))
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Abstract

Collagen, which accounts for one-third of human protein, is reduced due to human aging, and much attention is focused on making collagen into food to prevent such aging. Gel permeation chromatography with Reflective Index (RI) detec-tion (GPC/RI) was chosen as the most suitable instrument to confirm molecular weight distribution, and we explored the use of this technique for analysis of collagen peptide molecular sizes and distributions. Data reliability was verified by matrix-assisted laser desorption/ionization coupled to time-of-flight (MALDI-TOF) mass spectrometric analysis. The data were considered meaningful for comparative analysis of molecular weight distribution patterns.

keywords
Collagen, GPC, molecular weight distribution, refractive Index (RI)


Submission Date
2021-08-02
Revised Date
2021-09-06
Accepted Date
2021-09-17
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Mass Spectrometry Letters